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Production of Lentiviral Vectors for Transducing Cells from the Central Nervous System
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JoVE Journal Neurociência
Production of Lentiviral Vectors for Transducing Cells from the Central Nervous System

Production of Lentiviral Vectors for Transducing Cells from the Central Nervous System

DOI:
10.3791/4031-v

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08:46 min

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May 24, 2012

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, , ,
1Department of Neurology and Hope Center for Neurological Disorders,Washington University School of Medicine

Capítulos

  • 00:05Título
  • 01:24Packaging of Lentiviral Vectors
  • 03:28Concentration and Purification of the Vectors
  • 04:50Titration of the Vectors
  • 06:25Transduction of Neocortical Cultures
  • 07:20Transduction Results
  • 08:25Conclusion

Summary

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In this protocol we describe production, purification and titration of lentiviral vectors. We provide an example of lentiviral vector-mediated gene delivery in primary cultured neurons and astrocytes. Our methods may also apply to other cell types in vitro and in vivo.

Tags

Lentiviral VectorsTransducing CellsCentral Nervous SystemGene DeliveryCNSNeurological DiseasesTherapeutic ApproachesDividing CellsNon-dividing CellsTransgenesPackaging CapacityLow ToxicityBiosafetyEfficiencyReplication-defectiveSelf-inactivatingSIN Lentiviral VectorsPlasmidsLong Terminal Repeat (LTR)Strong PromoterHIV-1 Tat ProteinPackaging Signal (Ψ)Rev-responsive Element (RRE)Polypurine Tract (cPPT)Nuclear Import

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