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从中枢神经系统转导细胞的慢病毒载体生产
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Neurociência
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JoVE Journal Neurociência
Production of Lentiviral Vectors for Transducing Cells from the Central Nervous System
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从中枢神经系统转导细胞的慢病毒载体生产

DOI:
10.3791/4031-v

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08:46 min

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May 24, 2012

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, , ,
1Department of Neurology and Hope Center for Neurological Disorders,Washington University School of Medicine

Capítulos

  • 00:05Título
  • 01:24Packaging of Lentiviral Vectors
  • 03:28Concentration and Purification of the Vectors
  • 04:50Titration of the Vectors
  • 06:25Transduction of Neocortical Cultures
  • 07:20Transduction Results
  • 08:25Conclusion

Summary

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在这个协议中，我们描述了慢病毒载体的生产，提纯和滴定。我们提供了一个在原代培养的神经元和神经胶质细胞的慢病毒载体介导的基因传递的例子。我们的方法也适用于其他类型的细胞<em>在体外</em>和<em>在体内</em>。

Tags

Lentiviral VectorsTransducing CellsCentral Nervous SystemGene DeliveryCNSNeurological DiseasesTherapeutic ApproachesDividing CellsNon-dividing CellsTransgenesPackaging CapacityLow ToxicityBiosafetyEfficiencyReplication-defectiveSelf-inactivatingSIN Lentiviral VectorsPlasmidsLong Terminal Repeat (LTR)Strong PromoterHIV-1 Tat ProteinPackaging Signal (Ψ)Rev-responsive Element (RRE)Polypurine Tract (cPPT)Nuclear Import

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