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测绘细菌功能的网络和途径<em>大肠杆菌</em使用合成基因阵列
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JoVE Journal Biologia
Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays
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测绘细菌功能的网络和途径<em>大肠杆菌</em使用合成基因阵列

DOI:
10.3791/4056-v

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14:06 min

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November 12, 2012

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, , ,
1Department of Molecular Genetics,University of Toronto, 2Banting and Best Department of Medical Research, Donnelly Centre,University of Toronto, 3Department of Biochemistry, Research and Innovation Centre,University of Regina

Capítulos

  • 00:05Título
  • 01:40Constructing Non-essential Query Deletion in an Hfr Cavalli Donor Strain
  • 06:42Arraying E. coli F-Recipient Mutants for E. coli Synthetic Genetic Array (eSGA) Screens
  • 08:45High-density Strain Mating Procedure to Generate E. coli Double Mutants
  • 11:01Results: Representative Analyses of Genetic Interaction (GI) Scores for Determining Pathway-level Functional Relationships
  • 13:16Conclusion

Summary

Tadução automática

Tadução automática

系统，大型合成基因（基因或基因上位性）互动屏幕可用于，探索遗传冗余和途径串扰。在这里，我们描述了一种高通量的定量合成基因阵列筛选技术，被称为ESGA我们制定的，阐明互作关系，并探索遗传相互作用网络<em>大肠杆菌</em>。

Tags

Bacterial Functional NetworksPathwaysEscherichia ColiSynthetic Genetic ArraysPhenotypesPhysical InteractionsFunctional InteractionsGene-gene InteractionsGenetic InteractionsGI ScreensEpistatic DependenciesFunctional RelationshipsLarge-scale GI MapsProkaryotesFunctional AnnotationBacterial GenomesQuantitative Bacterial GI Screening MethodsE. Coli Synthetic Genetic Array (eSGA) Screening ProcedureGenome-scale Screening

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