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Funktionel Imaging af brunt fedt i mus med FDG micro-PET/CT
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JoVE Journal Biologia
Functional Imaging of Brown Fat in Mice with 18F-FDG micro-PET/CT

Funktionel Imaging af brunt fedt i mus med FDG micro-PET/CT

DOI:
10.3791/4060-v

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10:53 min

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November 23, 2012

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, ,
1Department of Translational Imaging,The Methodist Hospital Research Institute, Houston, 2Diabetes Research Center,The Methodist Hospital Research Institute, Houston

Capítulos

  • 00:05Título
  • 01:30Animal Preparation and Cold Treatment
  • 02:18Injection of FDG
  • 04:01Micro-PET/CT Imaging
  • 07:29Post-imaging Analysis
  • 08:54Results: FDG Uptake in Mouse BAT
  • 10:06Conclusion

Summary

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En fremgangsmåde til funktionel imagografi af mus brunt fedtvæv (BAT) er beskrevet som kold-stimuleret optagelse af 18F-fluordeoxyglucose (FDG) i BAT ikke-invasivt vurderes med en standardiseret micro-PET/CT protokol. Denne fremgangsmåde er robust og følsom til at påvise forskelle i BAT-aktiviteter i musemodeller.

Tags

Functional ImagingBrown FatMice18F-FDG Micro-PET/CTEnergy ExpenditureThermogenesisSympathetic Nervous SystemAdrenergic ReceptorBody Mass Index (BMI)Diabetic ParametersAnti-obesity/anti-diabetes TherapyEnergy BalanceCold Challenge-based Positron Emission Tomography (PET)Standardized ProtocolSmall Animal ModelsC57BL/6J MiceFasting Conditions18F-Fluorodeoxyglucose (FDG)Micro-PET/CT SystemPET ImagesCT Images

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