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绿色怪物承载多基因缺失的酵母菌株的产生过程

JoVE Journal
Biologia

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JoVE Journal Biologia

The Green Monster Process for the Generation of Yeast Strains Carrying Multiple Gene Deletions

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绿色怪物承载多基因缺失的酵母菌株的产生过程

DOI:

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13:06 min

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December 15, 2012

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Yo Suzuki, Jason Stam, Mark Novotny, Nozomu Yachie, Roger S. Lasken, Frederick P. Roth⁴

¹Department of Synthetic Biology and Bioenergy,J. Craig Venter Institute, ²Department of Microbial and Environmental Genomics,J. Craig Venter Institute, ³Donnelly Centre & Department of Molecular Genetics,University of Toronto, ⁴Lunenfeld Research Institute,Mt Sinai Hospital

Capítulos

00:05Título
01:38Sexual Cycling
06:13Flow Cytometry
09:26Genotyping
10:28Results: Yeast Strains Carrying Multiple Gene Deletions as Generated by the Green Monster Process
12:47Conclusion

Summary

Tadução automática

绿色妖怪方法能够快速组装的多个标记的报道基因编码绿色荧光蛋白的缺失。这种方法是基于对驾驶酵母菌株通过各种各样的缺失性和携带更多的缺失的细胞的荧光为基础的浓缩的重复循环。

Tags

Green Monster Process Yeast Strains Gene Deletions Phenotypes Genetic Background Environmental Condition Mutation Testing Hidden Gene Functions Genetic Interactions Multi-mutant Interactions Paralogs Saccharomyces Cerevisiae Gene Knockout Selectable Marker Homologous Recombination Marker Removal Green Monster Method Green Fluorescent Protein (GFP) Reporter

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