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复制和β细胞功能评估Adenovirally转分离的啮齿动物胰岛
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JoVE Journal Medicina
Assessing Replication and Beta Cell Function in Adenovirally-transduced Isolated Rodent Islets
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复制和β细胞功能评估Adenovirally转分离的啮齿动物胰岛

DOI:
10.3791/4080-v

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09:31 min

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June 25, 2012

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, , ,
1Department of Pediatrics,Indiana University School of Medicine, 2Department of Cellular & Integrative Physiology,Indiana University School of Medicine

Capítulos

  • 00:05Título
  • 00:58Adenoviral Transduction and Culturing of Rat Islets
  • 03:26Insulin Secretion Assay
  • 06:13Thymidine Incorporation Assay
  • 07:49Beta Cells Overexpressing “Gene #6”
  • 09:09Conclusion

Summary

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Tadução automática

该协议允许一个确定调节功能的β细胞质量的因素，找到治疗糖尿病的潜在的治疗靶点。该协议包括一个精简的方法来评估胰岛细胞复制和β细胞功能，在离体大鼠胰岛细胞与腺病毒基因表达操纵。

Tags

ReplicationBeta Cell FunctionAdenovirally-transduced Isolated Rodent IsletsGlucose HomeostasisInsulinGlucagonPancreatic Beta CellsAlpha CellsFunctional Beta Cell MassNutrient LoadDiabetesAutoimmune AttackType 1 DiabetesType 2 DiabetesInsulin SecretionMolecular PathwaysTherapeutic TargetsBeta Cell GrowthImpairing Beta Cell FunctionOncogenesTarget Genes

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