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Omdannelse af en ELISA til en Luminex xMAP assay under anvendelse af en multiplex antistof screeningsmetode
JoVE Journal
Biologia
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JoVE Journal Biologia
Conversion of a Capture ELISA to a Luminex xMAP Assay using a Multiplex Antibody Screening Method

Omdannelse af en ELISA til en Luminex xMAP assay under anvendelse af en multiplex antistof screeningsmetode

DOI:
10.3791/4084-v

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14:48 min

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July 06, 2012

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, , ,
1Chemistry Research and Development,Luminex Corporation, 2Global Marketing,Luminex Corporation

Capítulos

  • 00:05Título
  • 01:29Coupling Antibodies to MagPlex Microspheres, using the xMAP Antibody Coupling Kit
  • 04:58Antibody Screening
  • 07:20xMAP Functional Assay
  • 09:07ELISA Assay
  • 09:44Results: Comparison of an ELISA Assay for TNF-alpha Cytokine to the Converted Assay using the xMAP Platform
  • 14:28Conclusion

Summary

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Tadução automática

En ELISA let kan omdannes til en Luminex xMAP assay og gennem fordelene ved multipleksing flere antistoffer kan screenes samtidigt at identificere en optimal antistof par, hvilket resulterer i forøget følsomhed og dynamikområde, og samtidig reducere assay omkostninger.

Tags

ELISALuminex XMAP AssayAntibody ScreeningMultiplexCapture AntibodyAnalytesBiological SamplesLife Science ResearchClinical DiagnosticsLimitations96-well MicroplateSample VolumeAntigenNon-specific BindingBackgroundEnzyme-mediated AmplificationSensitivityTechnologyHigher ThroughputFlexibilityReduced CostWorkflowLuminex XMAP TechnologyMicrosphere Array PlatformMonoplex AssayProtein ApplicationsNucleic Acid Applications

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