Creating Defined Gaseous Environments to Study the Effects of Hypoxia on C. elegans

Emily M. Fawcett; Joseph W. Horsman; Dana L. Miller

doi:10.3791/4088

Journal

/

Biologia

/

创建定义的气体环境研究缺氧对 C。线虫</em

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Biologia

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JoVE Journal Biologia

Creating Defined Gaseous Environments to Study the Effects of Hypoxia on C. elegans

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创建定义的气体环境研究缺氧对 C。线虫</em

DOI:

10.3791/4088-v

•

11:07 min

•

July 20, 2012

•

Emily M. Fawcett², Joseph W. Horsman, Dana L. Miller

¹Department of Biochemistry,University of Washington, ²Molecular and Cellular Biology Program,University of Washington

Capítulos

00:05Título
01:27Construction of Environmental Chambers
02:56Connecting the Gas Supply to the Environmental Chamber
04:39Preparation of Samples for Viability Assay
07:32Rapid Harvest of Hypoxia-exposed Worms
10:11Results: The Effect of Hypoxia on C. elegans
10:44Conclusion

Summary

Tadução automática

本文详细介绍了如何使用连续流缺氧商会产生与定义的O浓度的大气 2了解生物反应下降Ø 2。该系统易于安装和维护，并具有足够的灵活性，以适应广泛的O 2浓度和模型系统

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