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Capítulos
- 00:05Título
- 01:36Expression and Purification of Recombinant Tagged Fusion Proteins in Bacteria
- 05:21Cell Culture and Cell Lysate Preparation
- 06:03In vitro Assembly of a CFTR-containing Macromolecular Complex (CFTR-PDZK1-MRP4)
- 08:23Representative Results
- 09:06Conclusion
Cystic fibrosis transmembrane conductance regulator (CFTR), een epitheliale chloride kanaal, is gemeld om te communiceren met verschillende eiwitten en belangrijke cellulaire processen te reguleren, onder wie de CFTR PDZ motief-gemedieerde interacties zijn goed gedocumenteerd. Dit protocol beschrijft methodes die we ontwikkeld tot een PDZ-afhankelijke CFTR macromoleculaire signaleren van complexe monteren<em> In vitro</em>.
Tags
In Vitro AnalysisPDZ-dependent CFTR Macromolecular Signaling ComplexesCFTRChloride ChannelTransepithelial Fluid HomeostasisCystic FibrosisSecretory DiarrheaCFTR Cl- ChannelPhysical InteractionsFunctional InteractionsTransportersIon ChannelsReceptorsKinasesPhosphatasesSignaling MoleculesCytoskeletal ElementsCFTR Carboxyl Terminal TailPSD95/Dlg1/ZO-1 (PDZ) MotifScaffold Proteins
