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加工的主要脑肿瘤组织的干细胞检测和流量排序
JoVE Journal
Medicina
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JoVE Journal Medicina
Processing of Primary Brain Tumor Tissue for Stem Cell Assays and Flow Sorting
DOI:

08:14 min

September 25, 2012

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Capítulos

  • 00:05Título
  • 01:30Culture of Brain Tumor Tissue
  • 03:21Tumorsphere Dissociation for Flow Cytometry
  • 04:36Surface Staining
  • 05:17Flow Cytometry Acquisition and Analysis
  • 06:00Self-renewal Assay
  • 06:26Single Cell RT-PCR
  • 07:08Results: Dissociated Brain Tumor Tissue Can Be Sorted for Stem Cell Assays
  • 07:42Conclusion

Summary

Tadução automática

人脑肿瘤发病机制,鉴定的脑瘤开始的细胞(BTICs),罕见的一个异质性肿瘤具有干细胞特性的细胞内,提供了新的见解。我们已调整了特定的文化条件,以丰富BTICs,我们经常使用流式细胞仪检测,以进一步丰富这些人群。对这些孤立的细胞自我更新的检测和RT-PCR单细胞的转录分析可以随后进行。

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