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Genetic Study of Axon Regeneration with Cultured Adult Dorsal Root Ganglion Neurons
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Neurociência
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JoVE Journal Neurociência
Genetic Study of Axon Regeneration with Cultured Adult Dorsal Root Ganglion Neurons

Genetic Study of Axon Regeneration with Cultured Adult Dorsal Root Ganglion Neurons

DOI:
10.3791/4141-v

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09:42 min

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August 17, 2012

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,
1Department of Orthopaedic Surgery,Johns Hopkins University School of Medicine, 2Solomon H. Snyder Department of Neuroscience,Johns Hopkins University School of Medicine

Capítulos

  • 00:05Título
  • 01:50Preparing Neuronal Culture Coverslips
  • 02:37Collecting Adult Mouse DRG Neurons
  • 04:12Preparing Adult Mouse DRG Neurons for Electroporation
  • 05:40Electroporation and Culturing of Neurons
  • 08:03Genetically Manipulated Adult Mouse DRG Cultures
  • 09:15Conclusion

Summary

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An in vitro model for genetic study of axon regeneration using cultured adult mouse dorsal root ganglion neurons is described. The method includes a re-suspension/re-plating step to allow axon re-growth from neurons undergoing genetic manipulation. This approach is especially useful for loss-of-function studies of axon regeneration using RNAi-based protein knockdown.

Tags

Genetic StudyAxon RegenerationCultured Adult Dorsal Root Ganglion NeuronsCentral Nervous System (CNS)Peripheral Nervous System (PNS)Mature NeuronsAxon GrowthHostile EnvironmentPeripheral Nerve InjuriesConditioning LesionSpinal Cord InjuryMolecular MechanismsCNS Axon Regeneration

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