Journal
/
Bioengenharia
/
Engineering Adherent Bacteria by Creating a Single Synthetic Curli Operon
JoVE Journal
Bioengenharia
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Bioengenharia
Engineering Adherent Bacteria by Creating a Single Synthetic Curli Operon

Engineering Adherent Bacteria by Creating a Single Synthetic Curli Operon

DOI:
10.3791/4176-v

•

15:28 min

•

November 16, 2012

•

, , , ,
1UMR CNRS 5557 Ecologie Microbienne, Université Lyon 1,Université de Lyon, 2Département Biosciences, INSA de Lyon,Université de Lyon, 3INSERM U758, Ecole Normale Supérieure de Lyon,Université de Lyon, 4Laboratoire de Génie Civil et Ingénierie Environnementale, INSA de Lyon,Université de Lyon

Capítulos

  • 00:05Título
  • 01:55Designing the Biobrick Synthetic Curli Operon
  • 06:06Quantifying and Visualizing Adherent Bacteria on Polystyrene
  • 09:01Visualizing Adherent Bacteria on Glass by Microscopy
  • 12:22Results: Increased Adherence of Engineered Bacteria
  • 14:28Conclusion

Summary

Tadução automática

Tadução automática

The design of a synthetic operon encoding both the secretory apparatus and the structural monomers of curli fibers is described. Overproduction of these amyloids and adherent polymers allows a measurable gain of adherence of the E. coli chassis1. Easy ways to visualize and quantify adherence are explained.

Tags

EngenhariaAdherent BacteriaSynthetic Curli OperonE. ColiPromoterVisualizationQuantificationBacterial AdherenceSynthetic BiologyBBa K540000 BiobrickTranscriptional SignalsTranslational SignalsCurli RegulationMetal-regulated PromoterQualitative AssessmentQuantitative AssessmentBiofilm Formation

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/4176?article_type=v

Procedure for Lung Engineering
check_url/pt/4176?article_type=v

Creating Two-Dimensional Patterned Substrates for Protein and Cell Confinement
check_url/pt/4176?article_type=v

Synthetic, Multi-Layer, Self-Oscillating Vocal Fold Model Fabrication
check_url/pt/4176?article_type=v

Analyzing Cellular Internalization of Nanoparticles and Bacteria by Multi-spectral Imaging Flow Cytometry
check_url/pt/4176?article_type=v

Synthetic Spider Silk Production on a Laboratory Scale
check_url/pt/4176?article_type=v

High efficiency, Site-specific Transfection of Adherent Cells with siRNA Using Microelectrode Arrays (MEA)
check_url/pt/4176?article_type=v

Density Gradient Multilayered Polymerization (DGMP): A Novel Technique for Creating Multi-compartment, Customizable Scaffolds for Tissue Engineering
check_url/pt/4176?article_type=v

Optical Detection of E. coli Bacteria by Mesoporous Silicon Biosensors
check_url/pt/4176?article_type=v

Nanomanipulation of Single RNA Molecules by Optical Tweezers
check_url/pt/4176?article_type=v

Tissue Engineering by Intrinsic Vascularization in an In Vivo Tissue Engineering Chamber

Read Article