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一个工具包，使水环境中的烃类转化
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Bioengenharia
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JoVE Journal Bioengenharia
A Toolkit to Enable Hydrocarbon Conversion in Aqueous Environments
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一个工具包，使水环境中的烃类转化

DOI:
10.3791/4182-v

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20:28 min

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October 02, 2012

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, , , , ,
1Department of Biotechnology,Delft University of Technology, 2Delft Center for Systems and Control,Delft University of Technology

Capítulos

  • 00:05Título
  • 02:33BioBrick Assembly
  • 03:37Alkane Converstion Resting Cell Assay, in vivo
  • 04:46Alkane Conversion Enzyme Assay, in vitro
  • 06:18Ethyl Acetate Hydrocarbon Extraction and Concentration Measurements
  • 07:40Alcohol/Aldehyde Dehydrogenase Activity Assay
  • 08:49pCaiF Characterization and Tolerance Assay
  • 10:26Homolog Interaction Mapping
  • 11:11Representative Hydrocarbon Conversion Results
  • 19:46Conclusion

Summary

Tadução automática

Tadução automática

一个可持续发展的自动调节修复石油污染的细菌系统的设计采用标准的可互换的DNA部分（BioBricks）。一个工程<em> E.大肠杆菌</em>菌株用于在有毒的水溶液环境中，通过β-氧化降解烷烃。来自不同物种的酶显示烷烃的降解活性。此外，增加的耐受性<em> N</em>己烷，通过引入从烷烃耐受菌的基因来实现。

Tags

Hydrocarbon ConversionAqueous EnvironmentsEscherichia ColiToolkitAlkanesBioBricksGene Expression RegulationSurvival In Toxic EnvironmentsAlkane Degradation PathwayAlkanolsAlkanalsAlkanoic-acidsβ-oxidation PathwayMedium-chain AlkanesCycloalkanesAlkane Hydroxylase SystemGordonia Sp. TF68Long-chain AlkanesGeobacillus ThermodenitrificansADHALDHEnzyme ActivityGlucose ConditionsSubstrate-regulated Promoter

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