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荧光激活细胞分选（FACS）自主神经祖细胞的分离，从胎鼠的内脏器官的优化程序

JoVE Journal
Neurociência

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JoVE Journal Neurociência

An Optimized Procedure for Fluorescence-activated Cell Sorting (FACS) Isolation of Autonomic Neural Progenitors from Visceral Organs of Fetal Mice

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荧光激活细胞分选（FACS）自主神经祖细胞的分离，从胎鼠的内脏器官的优化程序

DOI:

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12:07 min

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August 17, 2012

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Dennis P. Buehler, Carrie B. Wiese, S. B. Skelton, E. Michelle Southard-Smith

¹Division of Genetic Medicine, Department of Medicine,Vanderbilt University School of Medicine

Capítulos

00:05Título
02:05Dissection
04:34Dissociation of Subdissected Tissues
05:59Filtering Cell Suspension
07:04Preparing Samples for FACS
08:00Flow Cytometry
08:47Results: Purification of Neural Crest-derived Neuronal Progenitors from Fetal Mouse Tissues
11:32Conclusion

Summary

Tadução automática

一个优化的过程，净化神经嵴源性神经祖细胞从小鼠胚胎组织被描述。此方法采用荧光报告基因的表达，通过荧光激活细胞分选（FACS）分离的离散人口。该技术可以应用到隔离整个发展或从成人组织中的神经元亚群。

Tags

Fluorescence-activated Cell Sorting FACS Autonomic Neural Progenitors Visceral Organs Fetal Mice Neural Crest Autonomic Ganglia Laser Capture Micro-dissection Fluorescent Reporters Tyrosine Hydroxylase TH Gene Catecholamines Enteric Neuronal Progenitors Migration Fetal Intestine Pelvic Ganglia Neurons EGFP Transgene Construct

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