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一个的趋电含量为神经前体细胞迁移的动力学分析外部施加直流电场
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Neurociência
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JoVE Journal Neurociência
A Galvanotaxis Assay for Analysis of Neural Precursor Cell Migration Kinetics in an Externally Applied Direct Current Electric Field
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一个的趋电含量为神经前体细胞迁移的动力学分析外部施加直流电场

DOI:
10.3791/4193-v

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11:00 min

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October 13, 2012

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, ,
1Institute for Biomaterials and Biomedical Engineering,University of Toronto, 2Lyndhurst Centre,Toronto Rehabilitation Institute, 3Department of Surgery,University of Toronto

Capítulos

  • 00:05Título
  • 01:25Galvanotaxis Chamber Preparation
  • 05:30Live Cell Time-lapse Imaging
  • 08:55Kinematic and IHC Analysis of Differentiated and Undifferentiated NPCs
  • 10:35Conclusion

Summary

Tadução automática

Tadução automática

在这个协议中，我们将演示如何构建自定义室，允许应用程序的直流电场，使时间的推移成像的成人脑源性神经前体细胞转趋电性。

Tags

Galvanotaxis AssayNeural Precursor Cell MigrationExternally Applied Direct Current Electric FieldNeural Stem CellsNeuroregenerative StrategiesMobilization Of NPCsLesion SiteEndogenous PrecursorsPeriventricular RegionMechanisms Of NPC MigrationDirect Current Electric Fields (dcEFs)Galvanotaxis PhenomenonPhysiological Electric FieldsCell MigrationNormal DevelopmentWound Repair

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