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流式细胞仪分离纯化<em> S.酵母</em>合子

JoVE Journal
Biologia

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JoVE Journal Biologia

Flow Cytometry-based Purification of S. cerevisiae Zygotes

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流式细胞仪分离纯化<em> S.酵母</em>合子

DOI:

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15:09 min

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September 21, 2012

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Serendipity Zapanta Rinonos², Jeremy Saks, Jonida Toska, Chun-Lun Ni², Alan M. Tartakoff²

¹Department of Pathology,Case Western Reserve University School of Medicine, ²Cell Biology Program,Case Western Reserve University School of Medicine, ³Case Comprehensive Cancer Center,Case Western Reserve University School of Medicine

Capítulos

00:05Título
02:03Haploid Cell Growth
03:08Conducting a Cross
04:43Harvesting Preparations
05:57Flow Cytometry
09:01Preparation for DeltaVision Microscopy
11:05Representative Analysis of Zygote Purification
14:27Conclusion

Summary

Tadução automática

为了净化受精卵<em> S.酵母</em>，相反交配型的单倍体细胞的设计，以表达红色或绿色荧光蛋白，共同孵育，以允许合子形成，并分级的使用基于流式细胞术的协议。高度富集的部分，随后的“组学”的研究，恢复初始的后代，受精卵形态和系统的调查。

Tags

Flow Cytometry-based Purification S. Cerevisiae Zygotes Haploid Cells Diploid States Mating Type Stable Diploids Mitotic Divisions Zygote Formation Soluble Mating Factors Receptor-mediated Signaling Cascades Cell-cell Fusion Model For Progenitor Or Stem Cell Function Biochemistry Of Zygote Formation Purification Of Yeast Zygotes Sedimentation Properties Hypertonic Sorbitol

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