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An Orthotopic Bladder Tumor Model and the Evaluation of Intravesical saRNA Treatment
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JoVE Journal Medicina
An Orthotopic Bladder Tumor Model and the Evaluation of Intravesical saRNA Treatment

An Orthotopic Bladder Tumor Model and the Evaluation of Intravesical saRNA Treatment

DOI:
10.3791/4207-v

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08:43 min

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July 28, 2012

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, , , , ,
1Department of Urology and Helen Diller Comprehensive Cancer Center,University of California, San Francisco , 2Alnylam Pharmaceuticals, Inc.

Capítulos

  • 00:05Título
  • 00:23Intravesical Tumor Cell Injection
  • 04:00Bioluminescence Imaging
  • 04:45Ultrasound Imaging
  • 06:31Injection of saRNA
  • 07:45Conclusion

Summary

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Establishing an orthotopic bladder tumor model to evaluate antitumor effects of intravesically delivered saRNA and monitoring tumor growth by ultrasound and bioluminescent imaging.

Tags

Orthotopic Bladder Tumor ModelIntravesical SaRNA TreatmentBladder Cancer TreatmentSmall Activating RNAOrthotopic Xenograft Mouse ModelUrethral CatheterizationInhaled General AnestheticChemical BurnIntravesical Silver Nitrate SolutionBladder Glycosaminoglycan LayerHuman Bladder Cancer CellsKU-7-luc2-GFP CellsCatheterIn Vivo Bladder UltrasoundBioluminescent ImagingLipid Nanoparticles (LNPs)Tumor Growth Monitoring

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