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利用人类纤维原细胞线粒体表型的监测领域的帕金森氏病

JoVE Journal
Medicina

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JoVE Journal Medicina

The Use of Primary Human Fibroblasts for Monitoring Mitochondrial Phenotypes in the Field of Parkinson’s Disease

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利用人类纤维原细胞线粒体表型的监测领域的帕金森氏病

DOI:

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15:09 min

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October 03, 2012

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Lena F. Burbulla², Rejko Krüger²

¹German Center for Neurodegenerative Diseases,DZNE, ²Laboratory of Functional Neurogenomics, Department of Neurodegenerative Diseases, Hertie Institute for Clinical Brain Research,University of Tübingen

Capítulos

00:05Título
01:47Skin Biopsy and Culturing of Human Fibroblasts
03:29Preparation of Human Fibroblasts for Live Cell Imaging Microscopy
04:59Measurement of Mitochondrial Function
06:36Image Analysis of MMP-dyed Fibroblasts
08:18Measurement of Mitochondrial Morphology
09:25Image Analysis of Mitochondrial Morphology in Fibroblasts
11:15Measurement of Mitochondrio-lysosomal Colocalization
13:01Live Cell Images of Human Fibroblasts
14:43Conclusion

Summary

Tadução automática

成纤维细胞在帕金森氏症的致病基因携带突变的患者代表一个方便<em>体外</em>模型来研究疾病相关的表型。活细胞成像提供了学习的机会，在活细胞的形态和功能参数。在这里，我们描述了准备的人成纤维细胞的粒线体的表型及随后的监测。

Tags

Primary Human Fibroblasts Monitoring Mitochondrial Phenotypes Parkinson’s Disease Ageing Risk Factor Neurodegeneration In Vitro Models Molecular Pathways Ex Vivo Models Disease-associated Mutations Skin Biopsies Culturing Human Fibroblasts Microscopic Techniques Mitochondrial Function Mitochondrial Dynamics

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