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Capítulos
- 00:05Título
- 02:01Construction of Lentiviral Luciferase Reporters
- 02:28Production of Lentiviral Particles: Seed Cells (Day 1)
- 03:05Transient Transfection: CaPO4/DNA Precipitation (Day 2)
- 04:23Harvest Viral Particles (Days 3-4)
- 05:02Infection of 3T3 Cells (Day 3 Onward)
- 06:12Synchronization and Change to Recording Medium
- 07:02Bioluminescence Recording of Reporter Cells
- 07:26Data Analysis and Presentation
- 08:12Results: Phase-specific Circadian Reporters
- 09:47Conclusion
Los relojes circadianos funcionar dentro de las células individuales, es decir, son células autónomas. A continuación, se describen los métodos para la generación de células autónomas modelos de reloj de forma no invasiva, luciferasa tiempo real basado en la tecnología de bioluminiscencia. Células Reporter proporcionar sistemas manejables, modelo funcional para el estudio de la biología circadiana.
Tags
Cell-autonomous Circadian Clock RhythmsGene ExpressionLuciferase Bioluminescence ReportersMammalian Circadian ClocksSleep-wake CyclesLiver MetabolismEndogenous Circadian ClocksSuprachiasmatic Nucleus (SCN)Peripheral ClocksOscillatorsNegative Feedback MechanismCell-based In Vitro AssaysLocomotor ActivitySCN Explants Ex VivoCell-based ModelsClock FunctionTemporal ResolutionReal-time Bioluminescence RecordingFirefly Luciferase Reporter
