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Rapid Isolation of Viable Circulating Tumor Cells from Patient Blood Samples
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Bioengenharia
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JoVE Journal Bioengenharia
Rapid Isolation of Viable Circulating Tumor Cells from Patient Blood Samples

Rapid Isolation of Viable Circulating Tumor Cells from Patient Blood Samples

DOI:
10.3791/4248-v

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07:32 min

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June 15, 2012

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, , , ,
1Department of Biomedical Engineering,Cornell University, 2BioCytics, Inc., 3Carolina BioOncology Institute, PLLC

Capítulos

  • 00:05Título
  • 01:15Preparation of Halloysite Nanotube Solution
  • 01:48Coating of Microtube Inner Surface with Halloysite Nanotubes
  • 03:10Preparation of Microtubes for Cell Isolation
  • 04:05Preparation of Samples for Cell Isolation
  • 05:07Cell Isolation
  • 06:18Results: Isolation of Circulating Tumor Cells (CTCs)
  • 07:10Conclusion

Summary

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Circulating tumor cells are isolated from the blood of cancer patients without inflicting cellular damage. Isolation of tumor cells is accomplished using a bimolecular surface of E-selectin in addition to antibodies against epithelial markers. A nanotube coating specifically promotes cancer cell adhesion resulting in high capture purities.

Tags

CTC IsolationCirculating Tumor CellsViable CTCPatient Blood SamplesDisease ProgressionCTC ConcentrationPersonalized TherapiesCellular DamageLeukocytes ContaminationCTC EnumerationMicrofluidic DeviceE-selectin GlycoproteinEpithelial MarkersNanoparticle CoatingHalloysite Nanotubes

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