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To-og tre-dimensionelle Live-Cell Imaging af DNA skade responset Proteiner
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Biologia
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JoVE Journal Biologia
Two- and Three-Dimensional Live Cell Imaging of DNA Damage Response Proteins

To-og tre-dimensionelle Live-Cell Imaging af DNA skade responset Proteiner

DOI:
10.3791/4251-v

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10:24 min

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September 28, 2012

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, ,
1Department of Radiation Oncology,Virginia Commonwealth University, 2Department of Biochemistry & Molecular Biology,Virginia Commonwealth University, 3Department of Anatomy & Neurobiology,Virginia Commonwealth University, 4Massey Cancer Center,Virginia Commonwealth University

Capítulos

  • 00:05Título
  • 02:07Cell Preparation
  • 02:41Microscope Setup and Image Acquisition
  • 07:56Image Processing and Analysis
  • 09:02Results: 53BP1 Foci Formation
  • 09:41Conclusion

Summary

Tadução automática

Tadução automática

Denne protokol beskriver en metode til at visualisere en DNA-dobbeltstrenget brud signalering protein aktiveres som reaktion på DNA-skade såvel som dens lokalisering under mitose.

Tags

Two-dimensionalThree-dimensionalLive Cell ImagingDNA Damage Response ProteinsDouble-strand BreaksMutationsChromosomal AberrationsGenomic InstabilityDNA Damage ResponseCell Cycle ArrestApoptosisNonhomologous End-joiningHomologous Recombination RepairProteins53BP1P53-binding Protein 1Modified HistonesFociHistone ModificationsChromatin Rearrangement

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