/

/

使用LysoTracker检测及鉴别胚胎干细胞在胚胎细胞程序性死亡

JoVE Journal
Biologia

É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.

JoVE Journal Biologia

Use of LysoTracker to Detect Programmed Cell Death in Embryos and Differentiating Embryonic Stem Cells

Please note that all translations are automatically generated. Click here for the English version.

使用LysoTracker检测及鉴别胚胎干细胞在胚胎细胞程序性死亡

DOI:

•

12:44 min

•

October 11, 2012

•

Jennifer L. Fogel, Thu Zan Tun Thein, Francesca V. Mariani

¹Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research, Keck School of Medicine,University of Southern California

Capítulos

00:05Título
01:48LysoTracker Staining of Mouse Embryos
04:53LysoTracker Staining of Differentiating ES Cultures
06:51Mounting Samples for Imaging
07:44Viewing LysoTracker-stained Samples and Image Analysis
09:21LysoTracker Staining in Shh Null Embryos and Cultured Cells
12:10Conclusion

Summary

Tadução automática

我们提出了一个简单的协议，可视化地区的小鼠胚胎中的细胞程序性死亡（PCD）和鉴别胚胎干（ES）细胞培养，使用高度水溶性染料LysoTracker。

Tags

LysoTracker Programmed Cell Death PCD Embryos Differentiating Embryonic Stem Cells Apoptosis Caspase Enzyme Cascade Membrane Blebbing Nuclear Shrinking DNA Fragmentation Autophagy Immune Cells Non-professional Phagocytes Lysosomal Activity

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

6.5 DPC小鼠胚胎剖析

从小鼠胚胎干细胞的造血干细胞的推导

人类胚胎干细胞的推导Immunosurgery

胚胎干细胞衍生的内皮细胞治疗肢体缺血

人类胚胎干细胞冷冻和解冻

电子显微镜对胚胎的制备果蝇胚胎心管

小鼠胚胎生殖细胞的分离和推导

视频人类胚胎干细胞克隆生长的生物信息学分析

胚胎干细胞分化成少突胶质前体

人类胚胎干细胞畸胎瘤形成命运的映射

Read Article