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Bruk av LysoTracker å oppdage programmert celledød i Embryoer og Skille Embryonic Stem Cells
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Biologia
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JoVE Journal Biologia
Use of LysoTracker to Detect Programmed Cell Death in Embryos and Differentiating Embryonic Stem Cells

Bruk av LysoTracker å oppdage programmert celledød i Embryoer og Skille Embryonic Stem Cells

DOI:
10.3791/4254-v

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12:44 min

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October 11, 2012

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, ,
1Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research, Keck School of Medicine,University of Southern California

Capítulos

  • 00:05Título
  • 01:48LysoTracker Staining of Mouse Embryos
  • 04:53LysoTracker Staining of Differentiating ES Cultures
  • 06:51Mounting Samples for Imaging
  • 07:44Viewing LysoTracker-stained Samples and Image Analysis
  • 09:21LysoTracker Staining in Shh Null Embryos and Cultured Cells
  • 12:10Conclusion

Summary

Tadução automática

Tadução automática

Vi presenterer en enkel protokoll å visualisere regioner av programmert celledød (PCD) i mus embryoer og differensierende embryonale stamceller (ES) cellekulturer ved hjelp av en svært løselig fargestoff kalt LysoTracker.

Tags

LysoTrackerProgrammed Cell DeathPCDEmbryosDifferentiating Embryonic Stem CellsApoptosisCaspase Enzyme CascadeMembrane BlebbingNuclear ShrinkingDNA FragmentationAutophagyImmune CellsNon-professional PhagocytesLysosomal Activity

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