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Ex vivo Culturing of Whole, Developing Drosophila Brains
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Neurociência
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JoVE Journal Neurociência
Ex vivo Culturing of Whole, Developing Drosophila Brains

Ex vivo Culturing of Whole, Developing Drosophila Brains

DOI:
10.3791/4270-v

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08:39 min

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July 27, 2012

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, ,
1National Institute of Neurological Disorders and Stroke, 2National Human Genome Research Institute,National Institutes of Health, Bethesda, MD

Capítulos

  • 00:05Título
  • 01:22Culturing Larval Brains
  • 02:23Culturing Pupal Brains
  • 03:30Fixing, Staining, and Mounting Larval and Pupal Brains
  • 04:38Representative Brain Culturing Results
  • 08:03Conclusion

Summary

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This article describes a method by which one can mimic in vivo development of the Drosophila mushroom body in an ex vivo culture system.

Tags

Ex Vivo CulturingDrosophila BrainsChronic Genetic ManipulationsCell BiologyDevelopmentCentral Brain StructuresPharmacological InterventionsLive ImagingSubcellular CompartmentAxon Initial Segment (AIS)Neuron-specific Cyclin-dependent KinaseCdk5Pharmacological InhibitorsActin OrganizationCell-surface Protein Fasciclin 2MutantsRemodelingConnectionsEmbryonic Mushroom Body (MB)MetamorphosisLarva To AdultOlfactory Learning And Memory

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