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Time-lapse Fluorescence Imaging of Arabidopsis Root Growth with Rapid Manipulation of The Root Environment Using The RootChip
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Bioengenharia
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JoVE Journal Bioengenharia
Time-lapse Fluorescence Imaging of Arabidopsis Root Growth with Rapid Manipulation of The Root Environment Using The RootChip

Time-lapse Fluorescence Imaging of Arabidopsis Root Growth with Rapid Manipulation of The Root Environment Using The RootChip

DOI:
10.3791/4290-v

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13:54 min

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July 07, 2012

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, , , , , ,
1Department of Plant Biology,Carnegie Institution for Science, 2Howard Hughes Medical Institute, 3Departments of Applied Physics and Bioengineering,Stanford University , 4Department of Microsystems Engineering (IMTEK) and Center for Biological Signaling Studies (BIOSS),University of Freiburg

Capítulos

  • 00:05Título
  • 03:24Step 1: Preparation of Growth Cones for Seed Germination
  • 03:58Step 2: Seed Germination and Seedling Growth
  • 05:02Step 3: Transfer of Seedlings onto the RootChip
  • 06:51Step 4: Connecting the RootChip to the Carrier
  • 08:11Step 5: Mounting the RootChip at the Microscope
  • 09:18Step 6: Operating the RootChip using the LabView Interface
  • 11:09Resultados
  • 12:43Conclusion

Summary

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This article provides a protocol for cultivation of Arabidopsis seedlings in the RootChip, a microfluidic imaging platform that combines automated control of growth conditions with microscopic root monitoring and FRET-based measurement of intracellular metabolite levels.

Tags

Time-lapseFluorescence ImagingArabidopsis Root GrowthRootChipRoot Environment ManipulationMicrofluidic DeviceNutrient UptakeSustainable ApproachesCrop YieldMicroscopic DetailPolydimethylsiloxane (PDMS)Micromechanical ValvesPerfusion SystemObservation ChambersTest SolutionRoot Biology

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