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在Live哺乳动物细胞中质粒复制多代荧光显微镜监测
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Biologia
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JoVE Journal Biologia
Monitoring Plasmid Replication in Live Mammalian Cells over Multiple Generations by Fluorescence Microscopy
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在Live哺乳动物细胞中质粒复制多代荧光显微镜监测

DOI:
10.3791/4305-v

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12:34 min

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December 13, 2012

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, ,
1Department of Oncology,University of Wisconsin – Madison

Capítulos

  • 00:05Título
  • 01:34Engineering of Cells with Visible Plasmids
  • 05:08Visualization of Labeled DNA
  • 09:33Post-acquisition Processing of Images
  • 10:33Results: Visualization of Plasmids in Live Mammalian Cells
  • 12:16Conclusion

Summary

Tadução automática

Tadução automática

观察单个活细胞中的DNA分子的方法，进行说明。该技术是基于对一个荧光标记的lac阻遏物蛋白质设计到感兴趣的DNA结合位点的结合。此方法可适于后续许多随着时间的推移在活细胞中的重组DNA。

Tags

Plasmid ReplicationLive Mammalian CellsFluorescence MicroscopyGamma-herpesvirusesEpstein-Barr VirusKaposi’s Sarcoma-associated HerpesvirusLicensed ReplicationViral ProteinCellular Replication MachineryCentromeresSouthern BlottingFluorescence In Situ Hybridization (FISH)Quantitative PCRVisualizing PlasmidsLactose Repressor ProteinLacO Sequence

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