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A Simple Protocol for Platelet-mediated Clumping of Plasmodium falciparum-infected Erythrocytes in a Resource Poor Setting
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
A Simple Protocol for Platelet-mediated Clumping of Plasmodium falciparum-infected Erythrocytes in a Resource Poor Setting

A Simple Protocol for Platelet-mediated Clumping of Plasmodium falciparum-infected Erythrocytes in a Resource Poor Setting

DOI:
10.3791/4316-v

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07:27 min

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May 16, 2013

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, , ,
1Malawi-Liverpool-Wellcome Trust Clinical Research Programme, 2Liverpool School of Tropical Medicine, 3Department of Microbiology, Division of Medical Parasitology,New York University School of Medicine

Capítulos

  • 00:05Título
  • 01:20Preparaing of Platelet-rich Plasma (PRP) and Platelet-poor Plasma (PPP)
  • 02:17Parasite Culture
  • 02:58Thin Blood Film Slide Preparation
  • 03:57Plasmagel Flotation
  • 05:01Clumping Assay
  • 06:08Results: Platelet-mediated Clump Assay
  • 06:57Conclusion

Summary

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This method investigates the platelet-mediated clumping phenotype of Plasmodium falciparum-infected erythrocytes (pRBC) in clinical isolates. This is performed by isolating and co-incubating platelet-rich plasma and a suspension of pRBC.

Tags

Platelet-mediated ClumpingPlasmodium Falciparum-infected ErythrocytesResource Poor SettingCerebral MalariaMicrovessels ObstructionP. Falciparum-parasitized Red Blood CellsSequestrationEndothelial CellsPost-capillary VenulesSplenic ClearanceLow Oxygen PressureCytoadherenceHost ReceptorsSevere Pathological Outcomes

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