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文化与偏振刺激人体肠道黏膜植的新方法

JoVE Journal
Biologia

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JoVE Journal Biologia

A Novel Method for the Culture and Polarized Stimulation of Human Intestinal Mucosa Explants

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文化与偏振刺激人体肠道黏膜植的新方法

DOI:

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06:38 min

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May 01, 2013

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Katerina Tsilingiri, Angelica Sonzogni, Flavio Caprioli, Maria Rescigno

¹Department of Experimental Oncology,European Institute of Oncology, ²Department of Pathology and Laboratory Medicine,European Institute of Oncology, ³U.O. Gastroenterologia 2, IRCCS Ca’ Granda,Ospedale Policlinico di Milano

Capítulos

00:05Título
01:19Obtaining and Preparing the Tissue
02:22Mounting the Tissue
03:38Stimulation
04:31Harvesting
05:11Mucosal Explants Responses to Pro-Inflammatory Stimulus
05:58Conclusion

Summary

Tadução automática

我们引入了一种新的方法，为维护人类文化和监测至少24小时应对各类刺激肠黏膜。有了我们的方法中，维持组织的极性，可用于生理刺激通过心尖路由。

Tags

Human Intestinal Mucosa Explants Intestinal Micro-environment Immunological Response Tolerance Against Food Antigens Immune Responses Against Pathogenic Microbes Microbiota-host Interactions Mucus Degradation Antimicrobial Peptides Epithelial Barrier Gut-resident Lymphoid Cells In Vitro Assays Mouse Models Irritable Bowel Syndrome Inflammatory Bowel Disease Colorectal Cancer

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