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Imunologia e Infecção
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Isolation of Lymphocytes from Mouse Genital Tract Mucosa
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Isolation of Lymphocytes from Mouse Genital Tract Mucosa

Isolation of Lymphocytes from Mouse Genital Tract Mucosa

DOI:
10.3791/4391-v

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04:46 min

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September 03, 2012

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,
1Department of Pathology and Laboratory Medicine, David Geffen School of Medicine,University of California, Los Angeles , 2California NanoSystems

Capítulos

  • 00:05Título
  • 01:12Removal of the Genital Tract
  • 02:15Digesting Genital Tract Tissue
  • 03:08Separating Genital Tract Cells Using Percoll Gradients
  • 03:59Representative Results
  • 04:22Conclusion

Summary

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An efficient way to isolate lymphocytes from mouse genital tract is described. This method takes advantage of enzyme digestion and Percoll gradient separation to allow efficient isolation. This technique is also adaptable to for use in other species

Tags

IsolationLymphocytesMouse Genital Tract MucosaMucosal SurfacesGastrointestinalUrogenitalRespiratory TractsPathogensVirusesBacteriaMucosaeImmunityPeyers PatchesReproductive Mucosal SitesSexually Transmitted Diseases (STD)Bacterial InfectionsViral InfectionsCenters For Disease Control And PreventionHealth Care SystemReproductive Mucosal ImmunityIsolating LymphocytesMurine Female Genital Tracts

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