É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Capítulos
- 00:05Título
- 02:43Treatment of Cells with Thrombin, RNA Isolation, Quality Assessment and Quantification of RNA
- 03:54Library Construction and Sequencing
- 08:22Data Analysis
- 12:16Results: RNA-seq Analysis Output
- 17:16Conclusion
Denne protokol udgør en komplet og detaljeret procedure at anvende RNA-seq, en kraftfuld næste generations DNA-sekventering teknologi, til at profilere transcriptomes i humane pulmonale mikrovaskulære endotelceller med eller uden thrombin behandling. Denne protokol er generalisere til forskellige celler eller væv ramt af forskellige reagenser eller sygdomstilstande.
Tags
RNA-seq AnalysisTranscriptomesThrombin-treatedControlHuman Pulmonary Microvascular Endothelial CellsGene ExpressionMRNA LevelsExternal SignalsDrug TreatmentHealthy StateDiseased StateNext-generation DNA Sequencing TechnologyRNA-sequencingCatalog TranscriptsTranscriptional StructureExpressed GenesChanging Expression LevelsComplete Transcriptome ProfilingDigital Type DatumCopy Number Of TranscriptGenomic SequenceProtocolHuman Pulmonary Microvascular Endothelial Cells With Thrombin Treatment
