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Kromosom Replikerer Timing Kombineret med Fluorescent<em> In situ</em> Hybridisering
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Biologia
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JoVE Journal Biologia
Chromosome Replicating Timing Combined with Fluorescent In situ Hybridization

Kromosom Replikerer Timing Kombineret med Fluorescent<em> In situ</em> Hybridisering

DOI:
10.3791/4400-v

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17:14 min

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December 10, 2012

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1Department of Biochemistry and Molecular Biology, Knight Cancer Institute,Oregon Health & Science University

Capítulos

  • 00:05Título
  • 01:53BrdU Incorporation: Terminal Labeling
  • 02:35Chromosome Harvest of Monolayer Cells Cultures
  • 05:53RNase Treatment and Ethanol Dehydration
  • 06:38Fluorescent Labeling BAC DNA: Nick Translation
  • 08:19In situ Hybridization
  • 12:18Capturing Images and Quantifying BrdU Incorporation
  • 14:27Results: Chromosome Replication Timing and FISH
  • 16:45Conclusion

Summary

Tadução automática

Tadução automática

En kvantitativ metode til analyse af kromosomreplikation timing er beskrevet. Fremgangsmåden anvender BrdU-inkorporering i kombination med fluorescerende<em> In situ</em> Hybridisering (FISH) til at vurdere replikation timingen af ​​mammale kromosomer. Denne teknik giver mulighed for direkte sammenligning af omlejrede og un-omlejrede kromosomer i samme celle.

Tags

Chromosome Replicating TimingFluorescent In Situ HybridizationDNA ReplicationS PhaseTemporal Replication ProgramDevelopmental CuesEpigenetic ModificationsX InactivationTranscriptional SilencingX Inactivation CenterChromosome RearrangementsCancer CellsIonizing Radiation

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