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在体外合成的原住民，纤维长间距“和”分段长间距胶原蛋白

JoVE Journal
Bioengenharia

This content is Free Access.

JoVE Journal Bioengenharia

In vitro Synthesis of Native, Fibrous Long Spacing and Segmental Long Spacing Collagen

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在体外合成的原住民，纤维长间距“和”分段长间距胶原蛋白

DOI:

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07:54 min

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September 20, 2012

•

Richard W. Loo², Jane Betty Goh², Calvin C.H. Cheng, Ning Su, M. Cynthia Goh²

¹Department of Chemistry,University of Toronto, ²Institute for Optical Sciences,University of Toronto

Capítulos

00:05Título
01:06Preparation of Native Collagen Fibrils
01:45Preparation of Fibrous Long Spacing Collagen
02:35Preparation of Segmental Long Spacing Collagen
03:19Atomic Force Microscopy Characterization of Collagen Fibrils
05:10Results: AFM Verification of Collagen Fibril Type
07:15Conclusion

Summary

Tadução automática

简单和可重复的过程进行了描述为三个组件从一个共同的商业可用的I型胶原蛋白单体结构不同的胶原蛋白。本机类型，纤维长的间距或节段性长间距胶原可以构造不同的条件，长为300nm，直径为1.4 nm的单体建筑块露出。

Tags

In Vitro Synthesis Native Collagen Fibrous Long Spacing Collagen Segmental Long Spacing Collagen Extracellular Matrix Structural Scaffolding Mechanical Strength Collagen Fibrils Banding Periodicity Type I Collagen Monomers Hierarchical Assembly Length Scales Microns Commercially Available Collagen Monomer Protocols Mixtures Of Structures Unbanded Fibrils Imaging Atomic Force Microscope

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