Journal
/
Neurociência
/
Ex vivo Live Imaging of Single Cell Divisions in Mouse Neuroepithelium
JoVE Journal
Neurociência
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Neurociência
Ex vivo Live Imaging of Single Cell Divisions in Mouse Neuroepithelium

Ex vivo Live Imaging of Single Cell Divisions in Mouse Neuroepithelium

DOI:
10.3791/4439-v

•

06:41 min

•

April 30, 2013

•

,
1Department of Human Genetics,Emory University School of Medicine, 2Department of Experimental Embryology,IGAB Polish Academy of Sciences

Capítulos

  • 00:05Título
  • 01:13Embryo Generation
  • 02:09Whole Mouse Embryo Culture and Viral Infection
  • 03:27Neural Tube Slice Preparation and Live Imaging
  • 05:14Representative Live Imaging Results
  • 06:20Conclusion

Summary

Tadução automática

Tadução automática

Here we develop the tools necessary for ex vivo live imaging to trace single cell divisions in the mouse E8.5 neuroepithelium

Tags

Ex Vivo Live ImagingSingle Cell DivisionsMouse NeuroepitheliumFluorescent MarkersCulturing SlicesEmbryonic Mouse NeuroepitheliumGenetic Cell Lineage TracingTamoxifen-inducible Cre LineCre Reporter LineDsRed ReporterCre-mediated RecombinationTamoxifen InductionIndividual Cell DivisionsTranscriptional ResponseSonic Hedgehog SignalingOlig2-eGFP Transgenic LineCilia MarkerSstr3-GFP ReporterNeuroepithelium ImagingTime-lapse Confocal ImagingPrimary Cilia FormationShh Response

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/4439?article_type=v

Multiple-mouse Neuroanatomical Magnetic Resonance Imaging
check_url/pt/4439?article_type=v

High-resolution Live Imaging of Cell Behavior in the Developing Neuroepithelium
check_url/pt/4439?article_type=v

Live Imaging of Mitosis in the Developing Mouse Embryonic Cortex
check_url/pt/4439?article_type=v

Two-Photon in vivo Imaging of Dendritic Spines in the Mouse Cortex Using a Thinned-skull Preparation
check_url/pt/4439?article_type=v

Ex Vivo Imaging of Postnatal Cerebellar Granule Cell Migration Using Confocal Macroscopy
check_url/pt/4439?article_type=v

Simultaneous ex vivo Functional Testing of Two Retinas by in vivo Electroretinogram System
check_url/pt/4439?article_type=v

Single-cell Resolution Fluorescence Live Imaging of Drosophila Circadian Clocks in Larval Brain Culture
check_url/pt/4439?article_type=v

Ex Vivo Imaging of Cell-specific Calcium Signaling at the Tripartite Synapse of the Mouse Diaphragm
check_url/pt/4439?article_type=v

Transpupillary Two-Photon In Vivo Imaging of the Mouse Retina
check_url/pt/4439?article_type=v

In vivo Calcium Imaging in Mouse Inferior Olive

Read Article