/

/

神经元对神经胶质细胞在微流控文化互动平台（MCP）的神经元轴突和神经胶质细胞共培养系统的影像学分析

JoVE Journal
Neurociência

É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.

JoVE Journal Neurociência

Imaging Analysis of Neuron to Glia Interaction in Microfluidic Culture Platform (MCP)-based Neuronal Axon and Glia Co-culture System

Please note that all translations are automatically generated. Click here for the English version.

神经元对神经胶质细胞在微流控文化互动平台（MCP）的神经元轴突和神经胶质细胞共培养系统的影像学分析

DOI:

•

09:34 min

•

October 14, 2012

•

Haruki Higashimori, Yongjie Yang²

¹Department of Neuroscience,Tufts University, ²Neuroscience Program,Tufts Sackler School of Graduate Biomedical Sciences

Capítulos

00:05Título
01:27Assembly of the Microfluidic Culture Chamber (MCP)
02:43Preparation of Neuronal Culture
03:33Induction of Neuronal Axons in Assembled MCP
04:48A Compartmentalized Co-culture System: Adding Cultured Astrocytes
06:15Results: Axon-induced GLT1 Promoter Activation in Astrocytes
09:07Conclusion

Summary

Tadução automática

本研究介绍了一种新的神经元轴突和神经胶质细胞共培养（ASTRO）平台的程序。在此共培养体系中的一个单一的轴突（及单一的神经胶质细胞）之间的直接相互作用，操纵变得可行，允许机械分析的相互神经胶质信令。

Tags

Imaging Analysis Neuron Glia Interaction Microfluidic Culture Platform MCP-based Neuronal Axon And Glia Co-culture System Signaling Pathways Central Nervous System (CNS)Neuron And Glia Mixed Cultures Neuronal Compartments Axon Dendrite Soma Culture System Soluble Factors Direct Membrane Contact Signals Single Axon Glial Cell

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

二层共培养小学的大鼠皮层神经元和神经胶质

微流控采用共培养开发设备牙胚支配分析

采用组合运用各种方法，定义质膜交付在轴突分支和神经元形态发生中的作用

间接神经元的星形胶质细胞共培养试验：一个<em>体外</em>设置 - 为神经元的神经胶质细胞相互作用的详细调查

激光诱导的斑马鱼视网膜变性及再生模型中的 m ü ller 胶质细胞活化

使用微流体腔系统在运动神经元培养物中有机细胞的斧头迁移

来自成人大鼠尿膀胱的原发神经元和胶质的隔离与培养

脑回路非侵入性断开的靶向神经元损伤

Dissection and Isolation of Murine Glia from Multiple Central Nervous System Regions

研究神经-胶质相互作用在缺血中的作用的细胞培养模型

Read Article