/
/
Imaging Analyse af Neuron til glia Interaktion i Mikrofluid Kultur Platform (MCP)-baseret Neuronal Axon og glia Co-kultur System
É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Capítulos
- 00:05Título
- 01:27Assembly of the Microfluidic Culture Chamber (MCP)
- 02:43Preparation of Neuronal Culture
- 03:33Induction of Neuronal Axons in Assembled MCP
- 04:48A Compartmentalized Co-culture System: Adding Cultured Astrocytes
- 06:15Results: Axon-induced GLT1 Promoter Activation in Astrocytes
- 09:07Conclusion
Denne undersøgelse beskriver procedurerne for at oprette en ny neuronal axon og (astro) glia co-kultur platform. I dette co-kultursystem, bliver manipulation af direkte interaktion mellem en enkelt axon (og enkelt gliacelle) mulig, således mekanistisk analyse af den gensidige neuron til gliale signalering.
Tags
Imaging AnalysisNeuronGlia InteractionMicrofluidic Culture PlatformMCP-based Neuronal Axon And Glia Co-culture SystemSignaling PathwaysCentral Nervous System (CNS)Neuron And Glia Mixed CulturesNeuronal CompartmentsAxonDendriteSomaCulture SystemSoluble FactorsDirect Membrane Contact SignalsSingle AxonGlial Cell
