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Capítulos
- 00:05Título
- 01:44Low Speed Extract Preparation
- 05:46Preparation of a DNA Damage-mimicking Structure (AT70)
- 06:31DNA Damage: MMS-treated Sperm Chromatin
- 07:35DNA Damage: UV-treated Sperm Chromatin
- 07:58Triggering the DNA Damage Checkpoint in LSE
- 08:54Induce DNA Damage Checkpoint in LSE with the AT70
- 09:35Results: DNA Damage Checkpoint of Xenopus Egg Extracts
- 10:05Conclusion
Xenopus ei extract is een nuttig modelsysteem om de DNA schade checkpoint onderzoeken. Dit protocol is voor de bereiding van Xenopus ei extracten en DNA schade checkpoint inducerende reagentia. Deze technieken kunnen worden aangepast aan een verscheidenheid van DNA beschadigende benaderingen in de studie van de DNA schade checkpoint signaling.
Tags
DNA Damage CheckpointXenopus Egg ExtractsEndogenous InsultsEnvironmental InsultsDNA Damage Checkpoint SignalingSensorsTransducersEffectorsATR (ATM And Rad3-related)KinasesChk1 (Checkpoint Kinase 1)Cell Cycle ArrestTranscription ActivationDNA Damage RepairApoptosisSenescenceGenomic InstabilityGenomic IntegrityHuman DiseasesCancer DevelopmentXenopus Laevis Egg ExtractsCell-free Extract Model System
