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皮肤纹身作为一种新的方法DNA疫苗交货

JoVE Journal
Bioengenharia
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JoVE Journal Bioengenharia

Skin Tattooing As A Novel Approach For DNA Vaccine Delivery

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皮肤纹身作为一种新的方法DNA疫苗交货

DOI:

10.3791/50032-v

•

06:37 min

•

October 18, 2012

•

Yung-Nung Chiu, Jared M. Sampson, Xunqing Jiang, Susan B. Zolla-Pazner³, Xiang-Peng Kong

¹Department of Biochemistry and Molecular Pharmacology,New York University School of Medicine, ²Department of Pathology,New York University School of Medicine, ³Healthcare System,Veterans Affairs New York Harbor

Capítulos

00:00Título
00:15Introduction
01:22Plasmid DNA Preparation
02:10Tattoo Equipment Setup
03:26Animal Skin Preparation
04:14DNA Tattooing
05:33Visualization of EGFP Expression
06:04Conclusion

Summary

Tadução automática

皮肤纹身是一个强大的和安全的方式来传递DNA疫苗皮内。在这里，一个DNA质粒EGFP交付纹身实验室小鼠的皮肤，和EGFP在皮肤细胞中的表达，然后通过激光共聚焦显微镜检查。

Tags

DNA Vaccine Nucleic Acid-based Vaccination Plasmid DNA Skin Tattooing Intradermal Delivery EGFP Confocal Microscopy Adjuvant Cost-effective Convenient

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