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1Department of Biochemistry and Molecular Medicine,University of California, Davis , 2NSF Center for Biophotonics Science & Technology,University of California, Davis , 3University of Tromsø, 4Department of Surgery (Division of Surgical Oncology),University of California, Davis , 5UC Davis Comprehensive Cancer Center,University of California, Davis , 6Department of Biological Chemistry,University of California, Davis
Capítulos
- 00:05Título
- 01:27Preparing Live Cell Specimens for Imaging
- 03:103D (Deconvolution) Fluorescence Microscopy
- 06:10Results: Quantitative Image-Based Analysis of Cells Undergoing Autophagy
- 09:02Conclusion
L'autophagie est un processus omniprésents qui permet aux cellules de se dégradent et recyclent des protéines et des organites. Nous appliquons la microscopie par fluorescence de pointe pour visualiser et quantifier les petits, mais les changements essentiels, physiques associés à l'induction de l'autophagie, y compris la formation et la distribution de autophagosomes et des lysosomes, et leur fusion dans autolysosomes.
