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Staphylococcus aureus Growth using Human Hemoglobin as an Iron Source
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Staphylococcus aureus Growth using Human Hemoglobin as an Iron Source

Staphylococcus aureus Growth using Human Hemoglobin as an Iron Source

DOI:
10.3791/50072-v

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06:37 min

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February 07, 2013

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, ,
1Department of Pathology, Microbiology and Immunology,Vanderbilt University Medical School

Capítulos

  • 00:05Título
  • 01:13Purification of Hemoglobin from Fresh Blood
  • 03:41Staphylococcus aureus Growth using Hemoglobin as a Sole Iron Source
  • 04:55Results: Human Hemoglobin as an Iron Source for S. aureus
  • 06:16Conclusion

Summary

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Here we describe a growth assay for Staphylococcus aureus using hemoglobin as the sole source of available nutrient iron. This assay establishes the role of bacterial factors involved in hemoglobin-derived iron acquisition.

Tags

Keywords: Staphylococcus AureusHemoglobinIron AcquisitionIsd SystemHemeIron-regulated Surface DeterminantGrowth AssayIron-deplete MediumBacterial Pathogen

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