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Biologia
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Isolation and Culture of Individual Myofibers and their Satellite Cells from Adult Skeletal Muscle
JoVE Journal
Biologia
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JoVE Journal Biologia
Isolation and Culture of Individual Myofibers and their Satellite Cells from Adult Skeletal Muscle

Isolation and Culture of Individual Myofibers and their Satellite Cells from Adult Skeletal Muscle

DOI:
10.3791/50074-v

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11:57 min

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March 22, 2013

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, ,
1Sprott Center for Stem Cell Research,Ottawa Hospital Research Institute, 2Department of Cellular and Molecular Medicine,University of Ottawa

Capítulos

  • 00:05Título
  • 01:44Preparations
  • 03:33Muscle Dissection and Digestion
  • 07:00Single Myofiber Dissociation and Culture
  • 09:35Ex vivo Expression of Pax7 and Myf5 in Myofibers
  • 11:02Conclusion

Summary

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Tadução automática

Isolation and culture of myofibers is the gold standard in vitro system to study the transition of satellite cells through quiescence, activation and differentiation. Importantly, the single myofiber culture system preserves the myofiber/stem cell association, which is an essential component of the muscle stem cell niche.

Tags

Myofiber IsolationSatellite CellsMuscle RegenerationBischoff ProtocolRosenblatt ProtocolMuscle Stem CellsSingle Myofiber CultureQuiescenceActivationProliferationDifferentiation

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