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T细胞实时成像实时信号复合物的形成

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Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

Real-time Live Imaging of T-cell Signaling Complex Formation

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T细胞实时成像实时信号复合物的形成

DOI:

10.3791/50076-v

•

10:31 min

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June 23, 2013

•

Elad Noy*¹, Maor H. Pauker*¹, Mira Barda-Saad

¹The Mina and Everard Goodman Faculty of Life Sciences,Bar-Ilan University

Capítulos

00:05Título
01:28T-cell Spreading Assay Preparation
02:52T-cell Activation and Imaging
07:21Imaging Data Analysis
08:42Results: Live Imaging of SLP76 Deficient T-cells
09:48Conclusion

Summary

Tadução automática

我们描述一个活细胞成像的方法在T细胞活化过程中，提供了洞察蛋白质动力学。我们展示了结合使用的T细胞扩散法，激光共聚焦显微镜成像分析得到的定量结果，按照整个T细胞活化信号复合物的形成。

Tags

Keywords: T-cell Signaling Immune System T Lymphocytes T-cell Activation T-cell Antigen Receptor (TCR)Antigen Presenting Cells (APCs)Immune Synapse Protein-protein Interactions Live Cell Imaging Protein Complex Formation Real-time Imaging Jurkat Cells Fluorescent Proteins

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