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Screening for Melanoma Modifiers using a Zebrafish Autochthonous Tumor Model
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JoVE Journal Medicina
Screening for Melanoma Modifiers using a Zebrafish Autochthonous Tumor Model

Screening for Melanoma Modifiers using a Zebrafish Autochthonous Tumor Model

DOI:
10.3791/50086-v

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10:23 min

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November 13, 2012

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, ,
1Program in Molecular Medicine and Department of Cancer Biology,University of Massachusetts Medical School, 2Departments of Surgery and Medicine,Weill Cornell Medical College , 3Departments of Surgery and Medicine,New York Presbyterian Hospital

Capítulos

  • 00:05Título
  • 01:47Screening for Melanoma Onset Modifiers
  • 03:46Tumor Invasion Assay
  • 04:38Antibody Staining
  • 06:23Transplantation Assay
  • 08:33Representative Melanoma Modifier Screening Results
  • 09:46Conclusion

Summary

Tadução automática

Tadução automática

A rapid way to screen for melanoma modifiers using a zebrafish autochthonous tumor model is presented. It takes advantage of the miniCoopR vector which allows for expression of candidate melanoma genes in melanocytes. A method to obtain melanoma-free survival curves, an invasion assay, a protocol for antibody staining of scale melanocytes and a melanoma transplantation assay are described.

Tags

Keywords: MelanomaZebrafishAutochthonous Tumor ModelGenomic StudiesMelanoma ModifiersMiniCoopR VectorMitfaBRAFV600EP53TransgenesisMelanoma InitiationMelanoma Cell Properties

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