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对黑色素瘤修饰符一个斑马鱼原地肿瘤模型的筛选

JoVE Journal
Medicina

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JoVE Journal Medicina

Screening for Melanoma Modifiers using a Zebrafish Autochthonous Tumor Model

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对黑色素瘤修饰符一个斑马鱼原地肿瘤模型的筛选

DOI:

10.3791/50086-v

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10:23 min

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November 13, 2012

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Sharanya Iyengar, Yariv Houvras³, Craig J. Ceol

¹Program in Molecular Medicine and Department of Cancer Biology,University of Massachusetts Medical School, ²Departments of Surgery and Medicine,Weill Cornell Medical College , ³Departments of Surgery and Medicine,New York Presbyterian Hospital

Capítulos

00:05Título
01:47Screening for Melanoma Onset Modifiers
03:46Tumor Invasion Assay
04:38Antibody Staining
06:23Transplantation Assay
08:33Representative Melanoma Modifier Screening Results
09:46Conclusion

Summary

Tadução automática

屏幕使用一个斑马鱼原地肿瘤模型的黑色素瘤的改性剂是一种快速的方式。它利用的miniCoopR允许候选黑色素的黑素细胞中的基因表达的载体。黑色素瘤生存率曲线，入侵检测，协议规模的黑素细胞抗体染色和黑色素瘤移植试验的方法获得。

Tags

Keywords: Melanoma Zebrafish Autochthonous Tumor Model Genomic Studies Melanoma Modifiers MiniCoopR Vector Mitfa BRAFV600E P53 Transgenesis Melanoma Initiation Melanoma Cell Properties

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