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Kombinerad Immunfluorescens och DNA FISH på 3D bevarade interfaskärnorna att studera förändringar i 3D Nuclear organisation
 
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Kombinerad Immunfluorescens och DNA FISH på 3D bevarade interfaskärnorna att studera förändringar i 3D Nuclear organisation

Article DOI: 10.3791/50087-v • 13:55 min • February 3rd, 2013
February 3rd, 2013 •
1, 1,2,3,4, 1
1Department of Pathology, New York University School of Medicine, 2New York University Center for Health Informatics and Bioinformatics, 3NYU Cancer Institute, 4Department of Pathology and Yale Cancer Center, Yale University School of Medicine

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Här beskriver vi ett protokoll för samtidig detektion av histon ändringar genom immunofluorescens och DNA-sekvenser med DNA-FISK följt av 3D mikroskopi och analyser (3D immuno-DNA FISH).

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Genetik molekylärbiologi bioinformatik Cancer Biology patologi medicinsk teknik immunologi intranukleär Space Nuclear Matrix fluorescens FISK 3D-DNA FISK DNA immunofluorescens immuno-FISH 3D mikroskopi Nuclear organisation interfaskärnorna kromatin ändringar
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