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A Visual Assay to Monitor T6SS-mediated Bacterial Competition
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
A Visual Assay to Monitor T6SS-mediated Bacterial Competition

A Visual Assay to Monitor T6SS-mediated Bacterial Competition

DOI:
10.3791/50103-v

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08:45 min

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March 20, 2013

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, ,
1MRC Centre for Molecular Bacteriology and Infection, Division of Cell and Molecular Biology,Imperial College London

Capítulos

  • 00:05Título
  • 01:55Bacterial Strains and Cultures
  • 03:04Competition Assay
  • 04:21Qualitative Observation of the Bacterial Killing
  • 06:44Representative Bacterial Competition Results
  • 07:41Conclusion

Summary

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Tadução automática

We describe a qualitative assay to monitor bacterial competition mediated by the Pseudomonas aeruginosa type VI secretion system (T6SS). The assay relies on the survival/killing of Escherichia coli target cells carrying a lacZ-reporter. This technique is adjustable to assess the bactericidal/bacteriostasis activity of T6SS-proficient microorganisms.

Tags

T6SSBacterial CompetitionVisual AssayHcpVgrGTseTsiPseudomonas AeruginosaAntimicrobial ActivityCFU CountingAntibiotic ResistanceScreening Tool

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