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单细胞测量液泡破裂引起细胞内病原体
JoVE Journal
Imunologia e Infecção
This content is Free Access.
JoVE Journal Imunologia e Infecção
Single Cell Measurements of Vacuolar Rupture Caused by Intracellular Pathogens
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单细胞测量液泡破裂引起细胞内病原体

DOI:
10.3791/50116-v

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10:39 min

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June 12, 2013

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, , , , , ,
1Dynamique des Interactions Hôte Pathogène,Institut Pasteur, Paris, France, 2Imagopole,Institut Pasteur, Paris, France, 3Pathogenomique Mycobacterienne Integrée,Institut Pasteur, Paris, France

Capítulos

  • 00:05Título
  • 01:29CCF4 Assay on Fixed Samples Using Shigella flexneri (96 Well Plates)
  • 05:31CCF4 Assay on Live Samples Using Shigella flexneri (35 mm Glass-bottom Format, MatTek)
  • 07:04Results: The CCF4-AM/β-lactamase Approach to Pathogens
  • 09:48Conclusion

Summary

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Tadução automática

我们描述了一种用于跟踪内膜破裂引起的细胞内的细菌<em>痢疾杆菌<em></em</em>和<em<em><em>结核杆菌</em></em</em>侵入宿主细胞后。我们的检测利用CCF4，宿主细胞质FRET探针在活的或固定的细胞。记者降解的酶活性存在于细菌表面。

Tags

Keywords: Single CellVacuolar RuptureIntracellular PathogensShigella FlexneriMycobacterium TuberculosisCCF4 FRET ReporterPhagosomal Membrane DisruptionReal-time ImagingHigh-throughput Analysis

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