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目标移植的肌肉祖细胞的分子成像
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JoVE Journal Medicina
Molecular Imaging to Target Transplanted Muscle Progenitor Cells
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目标移植的肌肉祖细胞的分子成像

DOI:
10.3791/50119-v

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09:24 min

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March 27, 2013

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, ,
1Imaging Program,Lawson Health Research Institute, 2Department of Anatomy and Cell Biology,Western University, 3Department of Medical Biophysics,Western University

Capítulos

  • 00:05Título
  • 02:17Culture and Transfection of C2C12 Myoblasts
  • 04:26Preparation of Myoblasts for Transplant
  • 05:16Cell Implantation
  • 06:49Injection of the Fluorescent Substrate
  • 07:13Bioluminescence Imaging to Image Luciferase-Expressing Myoblast Progenitor Cells
  • 07:37Results: Molecular Imaging to Target Transplanted Muscle Progenitor Cells
  • 08:42Conclusion

Summary

Tadução automática

Tadução automática

非侵入性的方法来评估成肌细胞移植的成功。该方法利用一个统一的融合的记者基因组成的基因的表达，可以被不同的成像方式成像。在这里，我们使用<em>的波动</em>报告基因序列到靶细胞通过生物发光成像。

Tags

Molecular ImagingMuscle Progenitor CellsDuchenne Muscular DystrophyBioluminescence ImagingPETSPECTMRIUltrasoundReporter GeneC2C12 MyoblastsMdx Mouse

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