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电穿孔轴突轨迹跟踪和突触目标的后脑在鸡胚

JoVE Journal
Neurociência

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JoVE Journal Neurociência

Electroporation of the Hindbrain to Trace Axonal Trajectories and Synaptic Targets in the Chick Embryo

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电穿孔轴突轨迹跟踪和突触目标的后脑在鸡胚

DOI:

10.3791/50136-v

•

10:04 min

•

May 29, 2013

•

Ayelet Kohl, Yoav Hadas, Avihu Klar, Dalit Sela-Donenfeld

¹Koret School of Veterinary Medicine,The Hebrew University of Jerusalem, ²Department of Medical Neurobiology,The Hebrew University of Jerusalem

Capítulos

00:05Título
01:17Egg Handling and Preparation
02:18Windowing, Injection and Electroporation
04:22Analysis of Embryos
06:18Cryo-sections and Immunofluorescence
07:14Representative Electroporation Results
09:26Conclusion

Summary

Tadução automática

神经网络是建立在胚胎大脑发育神经生物学的一个基本问题。在这里，我们结合电穿孔技术与新的遗传工具，如酶Cre / LOX-质粒和PiggyBac转介导的DNA换位系统在禽流后脑背中间贴上标签，并跟踪他们的轴突预测和突触在不同发育阶段的目标。

Tags

Electroporation Hindbrain Chick Embryo Axonal Trajectory Synaptic Target Neural Tube Cre/Lox PiggyBac GFP Interneurons DA1 Brainstem

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