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Real-time Analyses of Retinol Transport by the Membrane Receptor of Plasma Retinol Binding Protein
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Biologia
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JoVE Journal Biologia
Real-time Analyses of Retinol Transport by the Membrane Receptor of Plasma Retinol Binding Protein

Real-time Analyses of Retinol Transport by the Membrane Receptor of Plasma Retinol Binding Protein

DOI:
10.3791/50169-v

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14:32 min

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January 28, 2013

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, ,
1Department of Physiology, Jules Stein Eye Institute and Howard Hughes Medical Institute,University of California, Los Angeles

Capítulos

  • 00:05Título
  • 01:50Production, Refolding, and HPLC Purification of holo-RBP
  • 07:45Real-time Monitoring of STRA6-catalyzed Retinol Release and Retinol Loading
  • 10:16Real-time Monitoring of STRA6-catalyzed Transport of Retinol from holo-RBP to CRBP-I
  • 12:40Representative Data of Real-time Analysis of STRA6-catalyzed Retinol Transport
  • 13:47Conclusion

Summary

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Here we describe an optimized technique to produce high-quality vitamin A/RBP complex and two real-time monitoring techniques to study vitamin A transport by STRA6, the RBP receptor.

Tags

Retinol TransportPlasma Retinol Binding ProteinHolo-RBPSTRA6Real-time AnalysisElectrophysiologyCRBP-IVitamin A Transport Mechanism

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