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Evaluering af Polymeric Gene Delivery nanopartikler ved Nanopartikel Tracking Analyse og Højkapacitetsforskning Flowcytometri
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1Biomedical Engineering Department,Johns Hopkins University School of Medicine, 2Translational Tissue Engineering Center,Johns Hopkins University School of Medicine, 3Wilmer Eye Institute,Johns Hopkins University School of Medicine, 4Institute for Nanobiotechnology,Johns Hopkins University School of Medicine
Capítulos
- 00:05Título
- 01:21Cell Seeding and Transfection
- 04:34Nanoparticle Sizing and Plasmid per Particle Calculations
- 06:37Representative Data
- 08:01Conclusion
En protokol til nanopartikler sporing analyse (NTA) og højkapacitetsidentifikation flowcytometri for at evaluere polymere genafgivelsessystemer nanopartikler beskrives. NTA anvendes til at karakterisere nanopartikel partikelstørrelsesfordeling og plasmidet pr partikelfordeling. High-throughput flowcytometri muliggør kvantitativ transfektion Effektevalueringen for et bibliotek af gen-leveringsvehikler biomaterialer.
Tags
Keywords:
Polymeric Gene DeliveryNanoparticle Tracking AnalysisHigh-throughput Flow CytometryNon-viral Gene DeliveryCationic PolymersPolylysinePolyphosphoestersPoly(amidoamines)Polyethylenimine (PEI)Poly(beta-amino Ester)s (PBAEs)Human Retinal Endothelial Cells (HRECs)Transfection Efficiency96-well Plate Assay
